| Karakter | Acteur |
|---|---|
| James T. Kirk | William Shatner |
| Spock | Leonard Nimoy |
| Leonard McCoy | DeForest Kelley |
| Nyota Uhura | Nichelle Nichols |
| Montgomery-Scott | James Doohan |
| Hikaru Sulu | George Takei |
| Pavel Tsjekov | Walter Koenig |
Terugkerende tekens
| Karakter | Acteur |
|---|---|
| Christoffel Pike | Jeffrey Jager |
| Nummer één | Majel Barrett |
| Sarek | Mark Lenard |
| Amanda Grayson | Jane Wyatt |
| Scotty | James Doohan |
| Uhura | Nichelle Nichols |
| Tsjechov | Walter Koenig |
| Sulu | George Takei |
Gaststerren
| Karakter | Acteur |
|---|---|
| De Gorn-kapitein | Bill Erwin |
| Luitenant Janice Rand | Grace Lee Whitney |
| Luitenant Ayelborne | David Ziel |
| Luitenant Leslie | Eddie Paskey |
| Luitenant DeSalle | Michaël Ansara |
| Luitenant-commandant McGivers | Ron Howard |
| Luitenant Riley | Michaël Bos |
| Commandant Kor | John Colicos |
| Kang | Michaël Ansara |
| Kodos de Beul | Leonard Nimoy |
| Khan Noonien Singh | Ricardo Montalban |
| Harry Mudd | Roger C. Carmel |
| Sylvia Tilly | Maria Wiseman |
| Michaël Burnham | Sonequa Martin-Groen |
| As Tyler | Shazad Latif |
| Paul Stamets | Antonius Rapp |
| Jett Reno | Tig Notaro |
Extra tekens
| Karakter | Acteur |
|---|---|
| Yeoman Rand | Grace Lee Whitney |
| Verpleegsterskapel | Majel Barrett |
| Vaandrig Tsjechov | Walter Koenig |
| Vaandrig Sulu | George Takei |
| M'Benga | Boeker Bradshaw |
| Arex | Ted Cassidy |
| Balok | Clint Howard |
| Elaan van Troyius | Frankrijk Nuyen |
| Leila Kalomi | Majel Barrett |
| Losira | Susan Oliver |
| Miramanee | Barbara Luna |
| Shahna | Yvonne Craig |
| Vina | Susan Oliver |
| Zarabeth | Marj Dusay |